Summary
Type: SPE
Application #: 303350
Catalog#: 730063 - SPE Cartridges, Chromabond, Cyano, 3ml, PP, 500mg, 50/pk
Detection: HPLC
Conditions: Column conditioning: The column is activated by washing with 2 ml of water and 1 ml of 0,1 M HCl.
Sample application: The solution was passed slowly through the column by mild suction (<1 ml/min).
Column washing: The columns was washed with 2 ml of water and 1 ml of acetone . The column was dried under vacuum for 10 min.
Elution: An aliquot of 1 ml mixture consisting of methanol (100 ml), triethylamine (0,03 ml), and acetic acid (0,3 ml) was applied to the column. The liquid was allowed to pass through the column by gravity and finally drained completely by centrifugation at 2000 g for 1 min.
Evaporation: The eluent was evaporated to dryness under an air stream at 50°C.
Authors: Kobylinska M Kobylinska K
Source: Journal of Chromatography B 744 (2000) 207-212
Details
Legend: Recovery rates
The absolute recovery of domperidone from human plasma using this method was
83+/- 2,17%. The mean recovery of 0,2 µg/ml was 97,55+/-4,38% (n=6).